S7446
 |    |  |
 | SOD from bovine erythrocytes is a homodimeric non-covalently bound protein with two 16.3 kDa subunits of 151 amino acids. Each monomer has one intrachain disulfide and one free sulfhydryl, two copper atoms and two zinc atoms. |
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S7446
Sigma
Superoxide Dismutase from bovine erythrocytes
BioUltra, lyophilized powder, ≥4,500 units/mg protein, ≥97% (SDS-PAGE)
| Synonym: | SOD, Superoxide: superoxide oxidoreductase |
| CAS Number: | 9054-89-1 |
| Enzyme Commission (EC) Number: | 1.15.1.1 ( BRENDA | IUBMB ) |
| EC Number: | 232-943-0 |
| MDL number: | MFCD00132404 |
Description
| Biochem/physiol Actions | Catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. Plays a critical role in the defense of cells against the toxic effects of oxygen radicals. Competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of NO.1 SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice.2,3 |
| Unit Definition | One unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25 °C in a 3.0 mL reaction volume. Xanthine oxidase concentration should produce an initial ΔA550 of 0.025 ± 0.005 per min. |
Properties
| grade | BioUltra |
| assay | ≥97% (SDS-PAGE) |
| form | lyophilized powder |
| composition | Protein, ≥95% biuret |
| storage temp. | −20°C |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| Safety Statements | 22-24/25 |
| WGK Germany | 3 |
References
| Cited Reference | 1. Beckman, J.S., et al., Apparent hydroxyl radical production by peroxynitrite: implications for endothelial injury from nitric oxide and superoxide. Proc. Natl. Acad. Sci. U. S. A. 87, 1620-1624, (1990) |
| 2. Tilly, J.L., and Tilly, K.I., Inhibitors of oxidative stress mimic the ability of follicle-stimulating hormone to suppress apoptosis in cultured rat ovarian follicles. Endocrinology 136, 242-252, (1995) | |
| 3. Keller, J.N., et al., Mitochondrial manganese superoxide dismutase prevents neural apoptosis and reduces ischemic brain injury. J. Neurosci. 18, 687-697, (1998) | |
| Merck | Merck 13,9092 |
